FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48793097
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC96200.020.0710454
GACCGCG95800.019.9427595
ACCGCGT97050.019.6858966
TTCGACG17500.019.4169926
GAACGAT108650.017.99405345
CCGCGTT108200.017.653457
CGCGTTC110950.017.2970038
CAGACCG114150.017.151463
TCACGCT134900.016.377548
TAAACCG91800.016.22422611
GTCCGTT30950.015.6408051
ACGCTCA146900.015.42255610
CTCGTAT58450.015.2542891
CGACGAG22800.015.19628
TTCACGC150100.014.7040667
ATGCGCG21000.014.56996545
AACCGAT104150.014.42971913
CGTATAA29650.014.3448123
CTCGACG106500.014.34363144
CGTATCG26100.014.140383
Produced by FastQC (version 0.11.5)