FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44429828
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1928380.4340282388669162No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG1208770.27206272326780107No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1012470.22788069312354753No Hit
CCCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAG797510.17949878176435885No Hit
GTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCAC717310.1614478453529012No Hit
CCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAGG712710.16041250486047348No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC654810.14738071909708947No Hit
CCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCACGG638960.1438132958786156No Hit
CCCCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAA509500.11467521323737738No Hit
CTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCTGGGATAGAA498680.11223991233997124No Hit
CTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCC491820.11069590456213335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT427650.024.54479445
TTCACGC237600.018.872267
AGAACGA559550.018.77105544
AACGCTA246650.017.64169523
ACGCTCA260650.017.32417910
ACGCTAC251550.017.28014224
AAAACGC260300.016.73404921
CGCTACC259900.016.6903425
CACTCAG406750.016.6127571
GAAAACG265350.016.3731820
AAACGCT268300.016.25165422
AGACCGC644600.016.1412984
CCGGCTT188450.016.083822
CTACACG78650.016.02085145
ACCGCGT643300.015.96711356
CCGGTTT111600.015.8278962
CGCGTTC652150.015.73877058
CCGCGTT653800.015.7059347
GACCGCG654950.015.6354045
CACGCTC288000.015.6242989
Produced by FastQC (version 0.11.5)