FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44429828
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA614120.1382224572195058No Hit
CCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTC498970.11230518380579821No Hit
GGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCG494740.11135312070080489No Hit
CAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGG482210.10853294322903974No Hit
CTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCC475120.10693716842658045No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN471120.10603687234620851No Hit
ATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACC451670.10165918265539989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCTC70700.015.1763089
GTTCTAT236450.014.9334571
ACGGAAC114450.014.1731836
GTCAGGA190950.013.989681
CCAGACG127800.013.6120462
GACGGAA124700.013.2800125
CGGGACG69650.013.2439517
ACCTAGC38100.012.872996
ACGTCTC76800.012.6568421
CAGACGG136550.012.5749883
CGCTCTC86050.012.25986411
TTCTATA462900.011.7604372
AGACGGA152750.011.5783864
GTTGTCG21250.011.54364126
CAGCGCT94100.011.4993558
TCGGGAC82700.011.48052416
ATATAGC25150.011.450386
CGGAACC141750.011.4427457
TCTCGGG85300.011.42077614
GCAGCGC96450.011.3591237
Produced by FastQC (version 0.11.5)