FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51015407
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG582540.11418903312875657No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA537300.10532112387146102No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG535110.10489184179202961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT291450.013.7154823
AGCTTAG207900.013.63057345
ATAGGGG211400.013.52319638
GGAGGTC294700.013.4576384
GGCATAG214000.013.28528935
GAGGTCA343000.011.7604585
GGTCACT330800.011.6155327
ATATATA102350.011.5615116
TGGCATA248500.011.44991134
AGGTCAC347900.011.3032616
ACTCTCC344750.011.28233211
AACTCTA159050.011.26996945
GTCACTC346400.011.2418018
TATATAC96950.011.0916657
TCACTCT359650.010.9966559
GAGCTTA262100.010.97623144
CACTCTC359100.010.7816810
AGGGATC177600.010.7154292
CATAGGG276600.010.51457737
GCATAGG279300.010.259836
Produced by FastQC (version 0.11.5)