FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49811218
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT888230.17831926936618975No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG740410.148643223299619No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA498540.10008588828323772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT175500.026.19257445
AGAACGA239850.019.21220444
ATCTACG43000.016.42985522
TACGTAT45750.015.29450225
CCCCCCT52750.015.1906171
GTCCGTT38750.014.9282641
CCCGTAT29050.014.4891641
TTCGGCG51150.014.428344
AGACCGC322500.014.317694
CTCCGGT59400.014.2857421
AAGAACG325550.014.1200143
CAGACCG330650.014.0940863
GACCGCG333650.013.8322945
CGTACGC52900.013.7818985
ACCGCGT344100.013.4703846
CCCGCGT18750.013.4451171
ACTCAGA292900.013.40601352
CGTATTG53600.013.18036427
CCGTACG56350.013.0580764
CTCCGAT48100.013.00910951
Produced by FastQC (version 0.11.5)