FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48540872
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGA112700.020.363945
CGTTACG21900.019.41742521
CTCGACG132850.017.46147344
TAAACCG132750.017.32178111
ATCGAGC83700.016.66669842
AACCGAT140800.016.45929313
AGACCGC126800.016.200494
GTCCGTT36150.015.8141931
CCCAAAT212100.015.66272451
TCGGCGC60100.015.53668345
AAACCGA149650.015.440804512
ACCGCGT135350.015.1770976
TCTCGAC153150.015.11754643
GACCGCG135800.015.0936675
CAGACCG143850.014.3741093
TTAAACC161450.014.32625310
GATCGAA70300.014.24229516
AGATCGA70000.014.23910715
ACCGATG162450.014.19651314
CCCCCCT43600.014.1444381
Produced by FastQC (version 0.11.5)