FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43673024
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA833790.1909164797015201No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG712310.16310068201368422No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG628050.14380730768723504No Hit
GTTTAAACAGTTCAGTGATCTTTAGTGCATTGTTTATGTGTGGGTTTCTCT588550.13476282292703157No Hit
CTTTATTTCCCTTCTTTTTCTCTTTAGCTCGGCTTATTCCAGGGGTGTGTT545440.12489174095203484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGC67300.023.7986186
GTTGTCG74050.020.30761126
TATAGCT83000.019.3430637
GCTAAGT81950.019.23193614
TGTCGTT82500.018.03114528
CAATATA97800.017.3485894
TTGTCGT91300.016.57940927
GTCGTTC91150.016.2458929
ACAATAT108450.015.8524163
GGAGGTC297100.015.5344064
TAAGTGC109050.014.96819116
GGGAGGT310800.014.8641333
TCGTTCC101700.014.6714130
AATATAG138500.014.0686845
GCTTAAA106550.013.95592641
CTAAGTG114750.013.93066315
GGTCACT339450.013.2946447
GTCACTC338850.013.2314188
GAGGTCA363450.013.0190715
CGGCTTA214150.013.00873930
Produced by FastQC (version 0.11.5)