FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47338271
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGA87550.018.8121945
CTCCGGT76850.017.6610161
CTCGACG97150.017.02262944
ATCGAGC84950.016.8452642
TCGGCGC46400.016.82658245
GTCCGTT37350.016.6928841
CGCAATC69800.016.2167722
TAAACCG101900.016.1184411
TTCGGCG51450.015.26237744
CGTACGC50500.015.2374495
AGACCGC47250.015.1903014
CCGTACG51650.014.9853074
CTCGGTT82300.014.9052381
CCCGTAT35250.014.7501051
AACCGAT111250.014.72334913
CTCCGAT56000.014.5901861
AAACCGA113400.014.56321712
CCCCCCT47250.014.4815151
GACCGCG49500.014.4543795
ACCGCGT49600.014.4252376
Produced by FastQC (version 0.11.5)