FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47338271
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGT84550.011.8176341
CGGTTTA78600.011.79388238
ATAGGGG81900.011.26372638
TTCGTCG84800.010.9070793
TCGTCGA88150.010.6457834
TTTCGTC91050.010.5785322
CGTCGAG93250.010.3529395
GGCATAG90000.09.94811935
CGGCTTA86900.09.86326630
CATAGGG95350.09.60359537
GTCGAGA107650.09.09120756
AGCTTAG106150.09.07346245
GCATAGG100400.09.05223736
ATCGGTT103650.08.98543836
TGCCCGT97700.08.56795944
GGTTTAA110100.08.56306239
TCGGTTT110450.08.49435637
CACACGC117500.08.1972814
ACACGCC117650.08.1675385
GTCACTC153100.08.1117978
Produced by FastQC (version 0.11.5)