FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44006122
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACG19600.011.13157342
TACGGTT31800.010.47967635
TAATCGG64300.010.2874944
CGTAAGA48800.010.11668528
ATAATCG68700.010.0216313
TTACGGT33950.09.89267834
GGTAACG67850.09.61052917
AATCGGT70250.09.6067075
CTTAGAC82900.09.44144339
CCGTAAG54050.09.340363527
GTAACGA69600.09.17504518
CTAGCAT87250.09.0786571
CTTGACG200600.09.04864342
TAACCTC85200.08.96959933
TTGACGT204550.08.86292243
TGTTACG39200.08.79871532
TTCGTCG23000.08.6085213
CGGTTGG40200.08.55917937
GTAGCGT57850.08.55853245
CTCTTAG90950.08.43176537
Produced by FastQC (version 0.11.5)