FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49312797
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG141600.013.5194645
GGCATAG146500.013.13661435
ACCCGGC72050.011.9910675
ATAGGGG162600.011.90617338
CGGTTTA54350.011.51435238
ATAGCTC68450.011.51035839
GTTTCGT63350.011.2296811
TGGCATA172700.011.20883834
CATAGGG176100.010.94230237
GCATAGG180100.010.67330936
TCAGACG81950.010.2663942
ACGGACA82850.010.23740818
GGGAGGT263350.010.2342573
TTTCGTC63850.010.2172112
TCACCCG94300.010.2109193
ACTCTCC250050.010.0942411
GTCACTC244700.010.0843128
TAGCTCT79000.010.058740
GGAGGTC260300.09.9911934
CGGCTTA72300.09.99034130
Produced by FastQC (version 0.11.5)