FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50336081
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2961390.5883235129091595No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA801260.15918203882419849No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG729450.14491593018534757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT548450.026.40709545
GACCGCG609200.024.4023155
ACCGCGT610950.024.3802976
AGACCGC613050.024.2702224
CAGACCG620650.024.0346553
CCGCGTT623600.023.8568657
CGCGTTC627650.023.6921738
TCAGACC662400.022.7276172
CTCAGAC711050.022.074741
GCGTTCT675600.022.0706359
AGAACGA661900.021.95244244
CGTTCTC714050.021.00194510
AATACGG730750.020.00390232
TACGGAG731950.019.85122534
ATACGGA739950.019.73998633
AAGAACG739100.019.6229543
CAATACG754650.019.39720731
CACTCCC781800.019.14168424
CCTCTCA780300.019.08320819
CCCAATA773400.019.04622329
Produced by FastQC (version 0.11.5)