FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76457
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1220.15956681533410938No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1160.15171926703898925No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1140.14910341760728252No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1120.14648756817557582No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1110.14517964345972245No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1100.14387171874386911No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1070.13994794459630905No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1060.13864001988045568No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1060.13864001988045568No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1050.13733209516460232No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1050.13733209516460232No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1040.13602417044874898No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1030.13471624573289562No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1010.13210039630118892No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1010.13210039630118892No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1010.13210039630118892No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC980.12817662215362885No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC980.12817662215362885No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC970.12686869743777549No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC970.12686869743777549No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC960.12556077272192212No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC960.12556077272192212No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC960.12556077272192212No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC950.12425284800606877No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC950.12425284800606877No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.12294492329021542No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.12294492329021542No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.12294492329021542No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.12294492329021542No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.12294492329021542No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC930.12163699857436205No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC930.12163699857436205No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC930.12163699857436205No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC930.12163699857436205No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC930.12163699857436205No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC930.12163699857436205No Hit
GGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.1203290738585087No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.1203290738585087No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.1203290738585087No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.11902114914265535No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.11902114914265535No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.11902114914265535No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC900.11771322442680199No Hit
TTGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC900.11771322442680199No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC900.11771322442680199No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.11640529971094864No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.11509737499509529No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.11509737499509529No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.11509737499509529No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.11509737499509529No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.11509737499509529No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.11509737499509529No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.11509737499509529No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.11509737499509529No Hit
CACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.11509737499509529No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC870.11378945027924192No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC870.11378945027924192No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC870.11378945027924192No Hit
CAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.11248152556338857No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.11248152556338857No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.11248152556338857No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.11248152556338857No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.11248152556338857No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.11117360084753522No Hit
TGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.11117360084753522No Hit
CTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.11117360084753522No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.10986567613168186No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.10986567613168186No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.1085577514158285No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.1085577514158285No Hit
GTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.1085577514158285No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.1085577514158285No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.1085577514158285No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.1085577514158285No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.10724982669997515No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.10724982669997515No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.10724982669997515No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.10724982669997515No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.10724982669997515No Hit
GGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.10724982669997515No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.10724982669997515No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.10724982669997515No Hit
CGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.10594190198412179No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.10594190198412179No Hit
ATTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.10594190198412179No Hit
TCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.10594190198412179No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.10463397726826844No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.10463397726826844No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.10463397726826844No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.10463397726826844No Hit
CTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.10463397726826844No Hit
AGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10332605255241509No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10332605255241509No Hit
ACGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10332605255241509No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10332605255241509No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.10201812783656172No Hit
CGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.10201812783656172No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.10071020312070837No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.10071020312070837No Hit
CAGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.10071020312070837No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.10071020312070837No Hit
TTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.10071020312070837No Hit
TAGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.10071020312070837No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.10071020312070837No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.10071020312070837No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCCTA1100.0295.000032
GGACCTA950.0295.000032
TCGCCTA1050.0295.02
GTTGATT206.1474566E-8295.0295
AGTCCTA1000.0295.02
TCGCATA108.449606E-4295.0295
CACGGGA206.1474566E-8295.06
GAGTCCT500.0295.01
GGCACCT206.1474566E-8295.01
GGTCCTA1150.0295.02
CGACCTA1350.0295.02
CACGCCT255.2568794E-10295.01
AGGACCT400.0295.01
GGATCCT108.449606E-4295.01
CCTACGA108.449606E-4295.05
GCGCCCT255.2568794E-10295.01
CTAAGGG108.449606E-4295.06
TAGCCTA850.0295.02
GACCCTA1250.0295.02
AGAACCT206.1474566E-8295.01
Produced by FastQC (version 0.11.5)