FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41722104
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGA84400.018.98128345
AACGCTA59400.017.95475223
ATCGAGC70900.017.80344842
ACGCTAC61700.017.21251524
GAACGAT71050.017.00584645
TAAACCG96600.016.56015411
CTCGACG99650.016.18934844
CGCTACC65750.016.15227725
AACCGAT100300.015.97176913
GTCCGTT34900.015.9275931
AGACCGC77050.015.8864674
GACCGCG77650.015.820875
ACCGCGT77650.015.7918186
CTCCGGT52900.015.0175011
CAGACCG83500.014.9289243
AAACCGA109100.014.70411112
CCCAAAT167250.014.6938321
TCGGCGC42800.014.61467245
CGAGCTT89750.014.59069644
AAAACGC76000.014.15098721
Produced by FastQC (version 0.11.5)