FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41722104
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGT73050.011.4609541
TAGCACG26750.011.43831629
ATAGGGG72300.011.29727438
CGGTTTA67650.011.275537538
TTCGTCG71400.010.8118943
CGGCTTA78950.010.62926430
TTTCGTC79250.010.3088972
CGGCTAA83400.010.25158831
TCTATAG123950.010.1682783
GGCATAG82350.010.16393235
GTTCTAT128550.010.0493481
CTATAGT123850.010.0129134
TACTAGA123250.09.78512915
TAGTGTG126900.09.6793057
CATAGGG86500.09.59880537
GCACCGA126150.09.5959744
CGTCGAG85850.09.5163445
AGCTTAG89500.09.40201845
CGTGTAG125700.09.3797236
CACCGAA139150.09.2326345
Produced by FastQC (version 0.11.5)