FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48008143
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG852640.1776032036898407No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG540880.11266422031779068No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA481260.10024549376967153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGTC326250.016.9270534
GGGAGGT331700.016.860223
GTTGTCG35150.014.83671226
GAGGTCA383750.014.5308775
GGCATAG311350.014.40669835
AGCTTAG311250.014.20838145
AGGTCAC399750.014.0041836
TGGGAGG337400.013.8842332
ATAGGGG332350.013.85309338
GTCACTC401600.013.5022078
TGGCATA341850.013.2549734
GAGCTTA338300.013.21214544
ACTCTCC414850.013.16261511
CTGGGAG385700.013.1510281
GGTCACT420200.012.9847887
TCACTCT449700.012.2628469
CACTCTC443000.012.21982210
CGGCTTA89450.012.08495230
CATAGGG398400.011.38381837
GCATAGG407000.011.07406836
Produced by FastQC (version 0.11.5)