FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48652707
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1108530.2278454927492524No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT566900.11651972417485423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG150700.022.2926225
AGACCGC152150.022.272434
ACCGCGT151650.022.2271636
CAGACCG159400.021.3582293
TCACGCT312450.021.120248
CCGGCTT317850.020.8911152
TTCGACG20100.020.5985366
GCCGGCT290050.020.5043091
TTCACGC323850.020.3976177
CCGCGTT167150.020.3118827
CGCGTTC168450.020.2085538
CACGCTC336200.019.6550839
ACGCTCA342000.019.41391410
CGGCTTC373550.018.462753
AACGCTA361900.017.90487123
ACGCTAC363100.017.80232224
TCAGACC199600.017.518832
GAAAACG369050.017.32025120
CGCTACC373350.017.2713925
AAAACGC381250.017.01973221
Produced by FastQC (version 0.11.5)