FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50277468
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGA597790.11889819113404836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT95850.021.7361745
ACGTTCG37900.021.4902826
AGACCGC104600.020.090374
ACCGCGT107300.019.689686
TGCGCGT46300.019.1946545
TCGACGA152000.019.18339745
TCGGCGC68800.019.09802845
CAGACCG113600.018.6967643
CCGCGTT115100.018.3356347
ACCGCGA21250.018.31687245
GACCGCG117400.018.0340925
CTCGACG163450.017.99151644
TAAACCG163400.017.92747711
CGCGTTC119150.017.7498768
ACGCTAC90750.017.70201124
GTCCGTT34400.017.4679091
AGAACGA121300.017.41734144
AACGCTA91800.017.40141523
CCCAAAT235400.017.1133351
TTCGGCG80100.017.05034844
Produced by FastQC (version 0.11.5)