FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47712959
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT44950.022.5316451
ATCGAGC67600.018.67228342
TCGACGA66500.018.23709545
CTCCGGT63000.018.1124611
CTCCGTT87300.017.505141
AGACGCT149950.017.34573241
ACAACGG61150.016.9623442
CCCGTAT29550.016.6800251
CCCCGTT84750.016.332231
TTCGGCG46750.016.2193444
TCGGCGC45300.016.19227845
CCCCCCT39850.016.0963211
CCCCGCA149300.016.00437535
AAGCGGC152200.015.95105528
CTACCGG14050.015.85386312
TAAACCG77050.015.73953711
CAACGGG65850.015.54665343
CGTTACG13950.015.48371321
CCCGTTT129450.015.4390651
GACAACG69650.015.3768441
Produced by FastQC (version 0.11.5)