FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47712959
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT26650.016.5442611
ACCCGGC61550.013.6749625
TAGCACG33200.013.62196829
CGGCTTA70150.011.64292830
TCGTGTA40800.011.24947714
GGCATAG85300.011.23646535
TCACCCG80950.011.231933
CGGACAC75750.011.22672813
CGAGTTA41600.011.14141334
ATAGCTC72450.010.9939739
CATAGGG89600.010.79790737
ATAGGGG91650.010.75293838
AGCTTAG90550.010.70977445
GTCGTGT42700.010.64308713
CCGGACA81450.010.55086612
CGGACAG78200.010.47281219
CACCCGG84700.010.4423474
GCATAGG92600.010.37521236
ACGGACA87000.010.29279718
GTCGCCG50650.09.95082644
Produced by FastQC (version 0.11.5)