FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48012428
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGA76700.019.56606945
GTCCGTT36650.016.7649041
CTCGACG93750.016.10366844
GAACGAT94700.015.96586445
TAAACCG95300.015.51159511
AGACCGC96150.015.4208514
GACCGCG98250.015.0683455
ACCGCGT97850.015.0609616
CCCGTAT29850.014.8536891
CTACCGG14800.014.59464312
CTCCGGT60750.014.4117181
AACCGAT104900.014.37083213
ATCGAGC58250.014.36883342
CAGACCG104750.014.3495723
CGCGTTC103150.014.265688
CCGCGTT104200.014.2298957
CCGTACG40250.013.7512254
CGTACGC39850.013.7198755
CCCCCCT41500.013.3955731
TCTCGAC113450.013.22804943
Produced by FastQC (version 0.11.5)