FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48012428
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGT77400.011.5704771
TTCGTCG75200.011.4003483
CGGTTTA79550.010.60715338
CGGCTTA86100.010.37497330
TTTCGTC87950.010.310512
TCGTCGA85450.09.9275074
CGGCTAA55900.09.17735931
CGTCGAG100000.08.97790055
ATCGGTT96900.08.96274336
GTCGAGA107950.08.37793356
CATCGGT104300.08.37016335
ATAGGGG55750.08.27402438
TCGGCTT109500.08.24024929
GGTTTAA107700.08.23167739
TCGGTTT108300.08.16480937
ACACGCC108650.07.662565
CACACGC107650.07.6502924
AGCTCGG122600.07.635593426
GCTCGGC120900.07.575435627
CATAGGG61050.07.55527337
Produced by FastQC (version 0.11.5)