FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45598754
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATC37300.019.1969382
CTCGCGT8750.017.7634891
GTCCGTT35650.017.4396111
ACGTTCG15650.016.9616016
CTACCGG25850.016.27409712
CCGCTAT50100.016.096541
ATGCGCG16050.016.0001945
CCCATAT147750.015.9627041
TAAACCG57900.015.7747811
AGACCGC53200.015.6588184
CGTTACG10150.015.07461921
TCTACCG28050.014.99769811
ACCGCGT55200.014.9563696
TCGGCGC43600.014.82827945
ATCGAGC56400.014.67800242
CCCCCCT46700.014.2778261
TCGACGA63100.014.24422645
CTCCGGT62800.014.2402661
GGGATAA120600.014.0204365
CAGACCG60550.013.9825673
Produced by FastQC (version 0.11.5)