FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45598754
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC52850.013.5792065
CGGACAC68700.010.40710213
CGGACAG71350.010.24179319
ATAGCTC69600.010.17905639
TAGCACG23550.010.05163429
CGGCTTA63650.09.9882430
CCGGACA76500.09.66842612
CGGTTTA47700.09.28719238
TCACCCG85950.09.1623853
CGGCTAA36950.09.09096731
GTTTCGT54300.09.0760921
TTTCGTC53950.09.0084222
TTCGTCG50200.08.874583
TGCCCGT122650.08.76564144
GCGTCGT15450.08.58495811
ACGGACA89050.08.53436618
CGGCCCG92700.08.4159568
GACACGG91050.08.24785815
GCCCGGA91800.08.22905310
GTGGCGA101750.08.1376652
Produced by FastQC (version 0.11.5)