FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48726990
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1066220.21881507558747215No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG920100.1888275881600731No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA693170.14225586271591986No Hit
GCCGGTTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG621060.12745708282001414No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT521120.10694688918810703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTT147900.023.6760061
ACGTCCG26100.023.5344532
CCGGTTT166150.022.3586982
TTCACGC478100.021.596447
GCCGGCT275050.021.1584551
CCGGCTT344750.020.4678172
TCACGCT502650.020.4342358
CGGTTTC197750.019.6502083
GCACTAC105450.019.45913325
AGACCGC141450.019.4380764
GACCGCG142750.019.0245915
ACGCTCA562450.018.53733810
TTTCACG200900.018.5241556
ACCGCGT150950.018.125226
CACGCTC570750.018.0708249
TTCGACG19300.018.069966
TCGTATG34900.017.85805145
CGGCTTC407100.017.6587943
CAGACCG159400.017.4893763
CGTCCGC35300.017.46456733
Produced by FastQC (version 0.11.5)