FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48726990
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA914350.18764754399974223No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG890650.18278370980846548No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA668490.13719090795470848No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG576940.11840255267152762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT32850.019.86044911
GTTGTCG40100.016.43920726
GTCGCCG39350.016.18170544
TCGTGTA39750.016.07474914
GGAGGTC385000.015.9882034
GGGAGGT388250.015.9297263
AGCGTCG41750.015.41058110
ATATAGC54250.014.5154166
GTCGTGT42500.014.4522313
CGTCGTG44300.014.06760912
GGTCGCC46600.013.61651243
GTAGGGA48100.013.56479518
TGGGAGG369200.013.4720482
AGCTTAG355100.013.185060545
CACCGAA616700.013.0206745
CACCCGG84400.012.9064241
GCACCGA627400.012.74904644
GAGGTCA493150.012.7463945
ATAGGGG370100.012.73114438
GTTCTAT398800.012.7091431
Produced by FastQC (version 0.11.5)