FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50772249
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGA604550.11907095153496156No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG562870.11086174260273561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG118950.022.329945
ACCGCGT120500.022.173346
AGACCGC122700.021.8674454
CCGCGTT130600.020.4585577
TTCGACG21000.020.2415736
CGCGTTC133300.020.0272758
CAGACCG140400.019.3509353
TCACGCT180600.019.2403098
CGTATAA103900.019.0921653
AACGCTA175650.019.00533723
TCGACGA164100.018.85392845
ACGCTAC178150.018.73872424
CTCGTAT134800.018.475451
TTCACGC191800.018.105087
CGCTACC184400.018.09177625
TAAACCG170950.018.09032811
TCGTATA125350.017.798752
CCGGCTT200050.017.7631782
CACGCTC197800.017.567249
ACGCTCA203000.017.42745610
Produced by FastQC (version 0.11.5)