FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47062981
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGA73950.019.53368245
AACGCTA49000.018.36702323
ACGCTAC52600.017.15273924
GAACGAT120150.016.38594845
GACCGCG119700.016.2968435
GTCCGTT44400.016.2215121
CTCCGGT60550.016.2067781
ACCGCGT121150.016.0646326
CGCTACC56100.016.00239225
ATCGAGC72450.015.838531542
AGACCGC123400.015.8082364
CTCGACG97000.015.26297344
TCGGCGC42850.014.91264945
TAAACCG96050.014.78110211
CGTTACG17650.014.65978121
CCGCGTT135100.014.40584857
CAGACCG137700.014.3462433
CCCCGCT79200.014.2091851
AACCGAT101250.014.08863913
CGCGTTC139150.014.0027328
Produced by FastQC (version 0.11.5)