FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47062981
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG114500.012.7536138
AGCTTAG121300.012.26300545
GGCATAG122100.011.97543835
GTCACTC199850.010.7849218
GTTTCGT84000.010.7160481
TTCGTCG76750.010.2916063
ACCCGGG75300.010.1909192
CATAGGG143600.010.18414737
GGAGGTC227350.010.1656354
GGGAGGT226100.010.1421043
GGTCACT214850.010.1049717
CGGTTTA77950.09.84310638
TGGCATA150450.09.80844734
TTTCGTC83600.09.7982632
GCATAGG151100.09.69230136
AGGTCAC239350.09.597066
CACCCGG88650.09.4177921
TCGTCGA86500.09.2877644
TCACTCT244400.09.1692649
ACTCTCC238950.09.16204311
Produced by FastQC (version 0.11.5)