FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48546770
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG39650.017.87435726
ATATAGC55450.015.1362176
TGTCGTT50150.014.5357428
GCTAAGT56500.013.69871414
TATAGCT67150.012.699457
ATAGGGG118800.012.669888538
TTGTCGT60600.012.62319827
GGCATAG121550.012.290688535
AGCTTAG127700.012.23008945
AATATAG87850.012.0641385
GTCGTTC61550.011.880053529
GGGAGGT232950.011.7654733
GGTCACT216550.011.7307967
GTCACTC225150.011.6922728
TCGTTCC62900.011.66084630
CGTAGGG84550.011.389237
GCGTAGG86000.011.3018236
CAATATA89450.011.2951184
GGCGTAG86900.011.1847735
CGTTCCC68050.011.10898131
Produced by FastQC (version 0.11.5)