FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46986482
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGA614090.13069503692572684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGA141500.020.26505945
GTCCGTT39950.019.1990031
CTCGACG156350.018.31578644
GACGAAA62600.017.32159845
CCCGTAT31150.017.0911181
TAAACCG173150.016.9792711
CGTTACG17600.016.61439921
ATCGAGC91200.016.52631242
CTCCGGT57500.016.5169851
CCCAAAT253300.016.449331
CGCGCAT7050.016.319151
ACGCGTC9100.016.06783340
TCTCGAC181300.015.94414843
AACCGAT187550.015.77201313
GCACTAC69250.015.65649725
AAACCGA190000.015.56833912
CCCGTTT96150.015.5553861
GCCGATA24300.015.30716845
ATCTCGA191500.015.18887942
CCCCCCT38350.015.0000021
Produced by FastQC (version 0.11.5)