FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46986482
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTTA149850.013.10129730
CAGGGGT176300.011.44475840
ATAGGGG92400.011.41508238
TCGGCTT174850.011.3698829
GGCATAG95350.011.09773435
GGGGTGT186750.011.022161542
GCTCGGC179500.010.96802127
GTTTCGT101200.010.9668621
AGCTCGG182300.010.9655126
AGGGGTG189250.010.76947241
GGTGTGT190600.010.74050744
TAGCACG23850.010.58479829
TTCGTCG96350.010.5558773
CTCGGCT194150.010.21635228
TTTCGTC107950.09.8176132
TAGCTCG206450.09.73868525
GCATAGG108850.09.69475436
CATAGGG108850.09.60770837
CGGTTTA106050.09.56409738
GGGTGTG215950.09.56303543
Produced by FastQC (version 0.11.5)