FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51783725
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGA566610.10941854800905111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG162750.018.69133844
TCGACGA167000.018.10787645
TTCGACG18300.017.2137856
TAAACCG180600.017.11710211
CCCCCCT43950.016.491991
CGTTACG24500.016.3464721
CCCCGCT49700.015.8974231
AACCGAT198300.015.79347813
GAACGAT170850.015.76390845
TCTCGAC195600.015.5291943
CTCCGGT64000.015.4404721
AAACCGA202000.015.43736312
CTACACG92000.015.43214445
GTTCGAC20300.015.4078955
CGACGAG20600.015.3997768
GTCCGTT41200.015.3527141
GACCGCG180650.015.2588825
ACCGCGT183900.014.9883486
AGACCGC186600.014.8569154
TAAGCCG47950.014.73418742
Produced by FastQC (version 0.11.5)