FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46686008
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCG73000.011.5615273
GTTTCGT79850.011.2740461
TAGCACG15250.011.21306629
TTTCGTC78650.011.102952
CGGTTTA77350.011.05356938
TCGTCGA78750.010.9459444
ATAGGGG81050.010.49344338
CGTCGAG87400.010.2229365
CGGCTTA94250.010.16973930
GGCATAG84650.010.02037235
TCAGACG114250.09.4753292
GTCGAGA101400.09.3198866
CGTGGCG162500.09.293171
CATAGGG94850.09.274981537
AGCTTAG100600.08.56624945
CAGACGT126550.08.5544043
AGCTCGG126000.08.33966126
TCGAGAT128050.08.3112247
CTAGCAT97500.08.2867561
TTAACGC41600.08.27493832
Produced by FastQC (version 0.11.5)