FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49643649
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT893010.17988403713030846No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG760260.15314345647718203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG30300.023.9804276
CCGGCTT291150.022.4209612
GCCGGCT266350.022.0791591
TTCACGC296900.021.9576177
TCACGCT307900.021.2754468
CGGCTTC343650.019.9719963
ACGCTCA338650.019.62923610
GACCGCG230150.019.5571615
AGACCGC234600.019.5229434
CACGCTC334750.019.421129
GTTCGAC37650.019.3170835
ACCGCGT233600.019.2984266
CAGACCG244000.018.9277343
CGACGAG38350.018.3601388
GAAAACG250900.018.24677520
CCGCGTT249100.018.0705097
CGCGTTC253050.017.8239968
CGTTACG15500.017.27180321
CGGTATA57350.016.71085220
TCAGACC287750.016.6446632
Produced by FastQC (version 0.11.5)