FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47439180
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT907580.19131443671665488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT217700.020.98146645
AGACCGC249450.017.6518784
ACCGCGT251650.017.515096
CTCGACG91850.017.39293544
GACCGCG251650.017.3363295
CGTTACG25750.017.12594821
TCGACGA94750.016.8370245
CAGACCG263600.016.7651023
CCGCGTT264700.016.6345427
CGCGTTC267000.016.4827868
TAAACCG97450.016.30032711
AGAACGA283650.016.16646444
TCAGACC302700.015.4325232
TTCGACG16550.015.36226756
GCGTTCT300150.014.7672949
AACCGAT110850.014.43136513
AAGAACG328250.013.99722343
CTCAGAC358100.013.9270281
AATACGG329100.013.81701532
CCCCCCT39000.013.7919681
Produced by FastQC (version 0.11.5)