FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47439180
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG29850.013.19058926
GCGTCGT18450.011.70607911
AGCTTAG175750.011.32931845
GGGAGGT249800.011.1986233
ATAGGGG178350.011.07665938
GGAGGTC251650.010.8926044
GTTTCGT84600.010.8536141
GGCATAG178700.010.78958435
ATATAGC45700.010.4382516
CGGTTTA66550.010.4133438
TTCGTCG69150.010.2844443
TCGTCGA71150.010.185034
TTTCGTC77350.010.1834192
CGGCTTA85800.010.06944830
CGTCGAG74900.010.03508955
TGGCATA198400.09.89992834
TGTCGTT41250.09.87223528
GAGCTTA201800.09.81132944
CATAGGG214050.09.21872537
ACTCTCC293650.09.21663111
Produced by FastQC (version 0.11.5)