FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49037168
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT615510.12551907565298226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT155050.021.02775445
CGCAATC69200.017.6552222
GACCGCG178500.017.5712135
ACCGCGT179750.017.4862356
AGACCGC182400.017.3065324
AGAACGA192100.017.08920944
GTCCGTT38350.017.0771
CGTTACG14450.016.81612821
CAGACCG191750.016.6855853
CCGCAAT85600.016.3005371
CCGCGTT194050.016.2206577
CGCGTTC198800.015.8217748
CTCCGGT61450.014.9424641
CCGGTAA80400.014.9163532
TCGGCGC50950.014.79438345
AGGGATA114600.014.7249514
TAATTAT123200.014.6646119
ATAATTA122650.014.5835738
TTCGGCG53100.014.23766644
AAGAACG231100.014.19564743
Produced by FastQC (version 0.11.5)