FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41708583
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1278960.3066419206809304No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAAAAGAACGAT687060.16472868426146245No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG598110.14340213859578976No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA420590.10084015561017741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC508150.022.3694884
GACCGCG498150.022.2812335
ACCGCGT500600.022.1227446
CAGACCG515000.022.0632113
TCAGACC559200.021.3976352
CGCGTTC533100.021.0701278
CCGCGTT526050.021.0530917
GAACGAT559300.020.56077245
GCGTTCT557550.020.1865049
CTCAGAC642700.019.9703371
CGTTCTC608500.019.46851310
TTCACGC271800.017.9891857
TTCGACG16000.017.8596126
TCACGCT275950.017.7757248
ATCGCGT8900.017.4440526
AGAACGA663000.017.40594144
GTTCTCT708400.016.81513611
TAAACCG127400.016.7960111
TACGGGG229400.016.55589734
AATACGG683350.016.45938732
Produced by FastQC (version 0.11.5)