FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41708583
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG635090.15226841918844378No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA519470.12454750620513769No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG423620.10156662478799627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGC43400.015.552646
GTTGTCG39300.015.28563526
TGTCGTT46400.013.09238828
ATAGGGG125750.012.72119638
GTCGTTC47850.012.41350929
GCTAAGT52900.012.0368914
GGCATAG128300.011.97715435
TATAGCT57800.011.9115367
GGGAGGT178850.011.8289133
GGAGGTC178250.011.5658364
TAGCACG21250.011.117393529
CAATATA66150.011.0236724
TTGTCGT57600.011.01533327
TCGTTCC55850.010.99786630
AGCTTAG145300.010.85485345
CGGCTTA109200.010.75515830
GTCACTC184550.010.5824598
GCGTCGA22500.010.39998711
TGCGTCG23150.010.39950510
TGGCATA147150.010.36632534
Produced by FastQC (version 0.11.5)