FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49345630
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA696430.1411330648732218No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG665850.13493596089461216No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG588940.1193499809405615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGTC299150.013.4808374
GGGAGGT310400.013.1081033
TAGCACG57100.012.73594729
ACTCTCC313150.012.568150511
AGCTTAG255300.012.46437345
ATAGGGG257800.012.44971338
TCAGACG110800.012.416372
GGCATAG259250.012.38921735
AGGTCAC339750.012.1411556
GGTCACT331050.011.9305817
TGCCCGT161800.011.78621944
GAGGTCA353650.011.7338755
TCACTCT340150.011.6044199
GTCACTC347450.011.4774678
CACTCTC337300.011.43546910
AGGGATC188500.011.3529452
TGGCATA287950.011.21687934
CGAGTTA67100.011.15460234
GTTGTCG24900.010.75999426
GAGCTTA303150.010.63312944
Produced by FastQC (version 0.11.5)