FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45813982
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG24450.011.59385526
GTTTCGT109700.010.629891
TTTCGTC99500.010.2716082
TCGTCGA100600.010.1145834
TTCGTCG99200.010.0984893
CGTAACG30250.09.89280242
CGTCGAG106400.09.753275
CACACGC131700.09.315764
ACACGCC132850.09.2855995
CGGTTTA104550.09.0819438
TCTATAG263250.08.64551453
CGGCTTA115400.08.63715230
CTATAGT269500.08.4951254
CGTGGCG87150.08.4208491
CACGCCT144750.08.3315176
TGTCGTT33050.08.30487328
ACGCCTT150000.08.1441317
TTCTATA297300.08.1171872
CGTTAAC13150.08.04595851
TATAGTG289550.08.0309655
Produced by FastQC (version 0.11.5)