FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50963104
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG663940.13027856388025344No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT551250.10816648844622966No Hit
GGGGAAACAGAGTCGTGGAGGCTTTGAATCTCTCAGAAAAAAGGAAAGACA528740.10374956753026661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG127100.022.0546575
AGACCGC130050.021.7447324
ACCGCGT128950.021.7381426
CAGACCG142500.019.955523
CGTATAA54750.019.8880443
CCGCGTT143700.019.5224787
CGCGTTC145500.019.2655418
TCGACGA120100.018.0536845
CTCGTAT79150.018.0223481
GTCCGTT38000.017.585141
TAAACCG130700.016.79975311
GCCGGCT244700.016.7160221
TCACGCT231300.016.6126298
CTCCGGT76200.016.5941241
CTCGACG133750.016.44753344
TCGGCGC71650.016.40351145
TCGTATA73300.016.3287832
TCAGACC180850.016.2469122
CCGGCTT293300.015.9473522
GCGTTCT178550.015.7876449
Produced by FastQC (version 0.11.5)