FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51241761
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1159820.22634272854127713No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGAT717740.1400693469531619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT268300.024.1222691
TTCGACG31950.023.3119146
TCACGCT320100.022.3376858
CCGGCTT340700.021.6433812
ACGCTCA344100.021.2308610
CACGCTC339550.021.0448919
TTCACGC345550.020.7315657
AACGCTA337300.020.54507323
GAACGAT192200.020.31046345
ACGCTAC341400.020.2917524
CGCTACC346450.019.95700325
AAAACGC354350.019.58176621
CGGCTTC392250.019.4931143
ACCGCGT200050.019.2568846
AGACCGC202400.019.1555924
GAAAACG364700.019.0076720
GACCGCG204800.018.8432145
ATACGGG192650.018.72123333
AAACGCT374000.018.63114722
CCGCGTT208650.018.4286547
Produced by FastQC (version 0.11.5)