FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49115656
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT701500.14282614895747295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA140400.021.26482423
ACGCTAC141450.021.12401824
AGACCGC168800.020.0722434
ACCGCGT169400.019.9745866
CGCTACC150200.019.95336325
GAACGAT170400.019.60762445
GACCGCG173800.019.4818465
CGCGTTC174750.019.3626088
CAGACCG178550.019.2407743
CCGCGTT176650.019.1674587
TCACGCT165650.018.9730978
AAAACGC159200.018.89416721
ACGTTCG19150.018.7972626
GAAAACG166650.018.10342220
GCACTAC80550.017.93293225
TTCGACG19400.017.2793716
GTCCGTT39800.016.7934421
AAACGCT182000.016.6020522
CACGCTC193450.016.246549
ACGCTCA198800.016.19405410
Produced by FastQC (version 0.11.5)