FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48434377
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGAT819980.1692971089521808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT286950.022.00170345
AGACCGC346350.018.1892974
GACCGCG349400.018.0627145
CAGACCG350400.018.0514623
ACCGCGT353300.017.7041686
AGAACGA367550.017.20755844
TCGGCGC112650.016.57760445
CCGCGTT377250.016.5627087
ATACGGG239400.016.48472233
TACGGGG238700.016.46723634
CGCGTTC385350.016.226248
TCGACGA62750.016.20688445
TTCGGCG117000.015.84587444
GCGTTCT400850.015.6942289
TCAGACC413700.015.4155692
AAGAACG419450.015.03014743
AATACGG421200.014.90362932
CTCAGAC455000.014.731011
CAATACG436000.014.44411131
CGTTCTC440700.014.36698810
Produced by FastQC (version 0.11.5)