FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48434377
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACG26100.011.89754229
CGGCTAA153800.011.46996931
ACGGCTA153000.011.35353530
ATAGGGG90600.010.58016538
TTCGTCG64000.010.3009363
GTTTCGT74950.010.05671
TTTCGTC68100.010.0111642
CGGTTTA64650.09.95424938
GGCATAG94800.09.92111835
CGTGGCG149300.09.9012011
CGCTGAT193800.09.7987581
AAGCCGG152750.09.70669345
GTCACTC152650.09.6968388
TCAGACG93500.09.6739422
CGAGTTA34000.09.52956534
TCGTCGA72350.09.3608884
CATAGGG102850.09.29761237
GTTGTCG18900.09.28699726
CGGCTTA69300.09.286230
GGTCACT158400.09.27426057
Produced by FastQC (version 0.11.5)