FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51975934
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT703700.13538958241712404No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGA521330.1003021898557898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGA134300.019.88722845
GAACGAT181350.019.62856145
CTCGACG138650.019.3117344
CGTTACG21500.017.05785621
TAAACCG157000.016.98215311
ACCGCGT213150.016.6359276
GACCGCG212850.016.5009275
AGACCGC212600.016.4887544
ATCGAGC90650.016.35728842
TTCGACG18750.016.1997626
AACCGAT167400.015.96744713
CAGACCG222550.015.863933
CCGCGTT225200.015.7257457
TTCGGCG71050.015.42264944
AAACCGA173600.015.37122912
CGCGTTC231750.015.32988
CTCCGGT72400.015.324861
AGAACGA235250.015.15024644
TCGGCGC72900.015.00058945
CCCAAAT269000.014.97575951
Produced by FastQC (version 0.11.5)