FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51975934
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG146600.012.24681445
ATAGGGG150050.011.98120738
GGCATAG148850.011.97050835
GTTGTCG26600.011.50328626
GTTTCGT114350.011.3760261
TTCGTCG109100.011.1602513
CGGTTTA109150.010.98735638
CGGCTTA137150.010.74475130
TTTCGTC117300.010.6870682
TCGTCGA114800.010.68442254
CGTCGAG119750.010.5617195
GTCACTC235800.010.4389858
CATAGGG177650.010.08173137
GGTCACT241800.010.0504157
GGAGGTC256750.010.0454774
GGGAGGT263450.09.9438873
GCATAGG178050.09.93216636
GAGCTTA179350.09.9104144
TGGCATA181000.09.8196734
TCAGACG79550.09.4777672
Produced by FastQC (version 0.11.5)