FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44321185
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG577220.1302356875160265No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA481980.10874709238933931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACTC278150.014.4141358
ATAGGGG199650.014.28621838
GGCATAG201600.014.13687435
GGTCACT285550.013.9537817
AGCTTAG211850.013.67830145
GGAGGTC297950.013.6685944
GGGAGGT308000.013.5003753
ACTCTCC314950.012.73811111
TGGCATA225000.012.697103534
AGGTCAC327600.012.5687676
TCACTCT335200.012.2155949
CATAGGG237950.012.12847737
GAGCTTA239100.012.10982544
CACTCTC339650.011.80457110
GCATAGG250150.011.47404436
GAGGTCA370300.011.2894855
TCTCCCC369100.010.93662813
GTTGTCG22700.010.80428126
TAGGCTA79200.010.79524610
GCTCTGT387400.010.51288922
Produced by FastQC (version 0.11.5)